Journal: Cell chemical biology
Article Title: Stabilization of lncRNA GAS5 by a Small Molecule and Its Implications in Diabetic Adipocytes
doi: 10.1016/j.chembiol.2018.11.012
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Donors N/A Additional tissue ZenBio ™ N/A Chemicals, Peptides, and Recombinant Proteins Fmoc-protected amino acids Chem-impex N/A TentaGel resin RAPP Polymere MB250002 Rink Amide-MBHA resin GL Biochem N/A 1-Hydroxybenzotriazole (HOBt) Oakwood Chemical Cat #24755 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) Oakwood Chemical Cat #024810 N -ethylmorpholine (NEM) Oakwood Chemical A0358553 5,5-Dimethyl-1,3-cyclohexanedione Oakwood Chemical Cat #011151 4,4’-Dimethoxytrityl Chloride Oakwood Chemical Cat #00118 4-(Bromomethyl)benzoic acid AK-Scientific Cat # 6232-88-8 3-Mercaptopropionic Acid Oakwood Chemical Cat # 094485 Benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP) Oakwood Chemical 009017 Fluorescein isothiocyanate Chemodex Cat # 00860 Erythrocyte lysis buffer Stem Cell Technologies N/A ASC medium ZenBio ™ Cat# PM-1 Adipocyte Medium, AM1 ZenBio ™ N/A differentiation medium, DM2 ZenBio ™ N/A RNA-Bee ™ Tel Test, Inc N/A Nucleofector ® solution Lonza N/A Critical Commercial Assays Omniscript ™ kit Qiagen N/A Glucose Uptake Fluorometric assay kit Sigma MAK084 Biotin Chromogenic Detection kit ThermoFisher N/A Experimental Models: Cell Lines adipose stem cells (ASC) ZenBio ™ N/A Oligonucleotides GAS5 sense primer 5’- CTTCTGGGCTCAAGTGATCCT -3’ anti-sense 5’ TTGTGCCATGAGACTCCATCAG -3’ This paper N/A IR-A sense primer 5’- GTTTTCGTCCCCAGGCCATC -3’ and anti-sense 5’ CCAACATCGCCAAGGGACCT -3’ This paper N/A IR-B sense primer 5’- CACTGGTGCCGAGGACCCTA -3’ and anti-sense 5’ GACCTGCGTTTCCGAGATGG -3’ This paper N/A IR promoter set 1 sense primer 5’- AGATCTCTGGCCATTGC ACTCCAG -3’ and anti-sense 5’ TTCAATAAACAGTTTGCTA GGAGC -3’ This paper N/A IR promoter set 2 sense primer 5’- TCGAGTCACCAAAATAAACAT -3’ and anti-sense 5’ TGCAGGGGAGGGAGGTGCCGC -3’ This paper N/A GAPDH sense primer 5’- TGACGTGCCGCCTGGAGAAAC -3’ and anti-sense 5’- CCGGCATCGAAGGTGGAAGAG -3’; This paper N/A CAT sense: 5’ GCCGCTGGCGATTCAG 3’ and antisense 5’ TTCATTAAGCATTCTGCCGACAT 3’ This paper N/A UPF1 sense: 5’ AGATCACGGCACAGCAGAT 3’ and antisense 5’ TGGCAGAAGGGTTTTCCTT 3’ This paper N/A U1snRNA sense: 5’ TCCCAGGGCGAGGCTTATCCATT 3’ and antisense 5’ GAACGCAGTCCCCCACTACCACAAAT 3’ This paper N/A Software and Algorithms Compass software ProteinSimple https://www.proteinsimple.com/compass/downloads/ Excel ™ Microsoft Office https://office.microso1tcom/excel/ PRISM ™ Graphpad Software https://www.graphpad.com/scientific-software/prism/ OriginPro OriginLab https://www.originlab.com/ Open in a separate window KEY RESOURCES TABLE ncRNA GAS5 regulates expression of insulin receptor in adipocytes.
Techniques: Recombinant, Lysis, Software
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